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illuminator microled (brightfield)  (Carl Zeiss)


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    Structured Review

    Carl Zeiss illuminator microled (brightfield)
    Schematic of the closed-loop neural recording setup for sleep/wake response tracking Video recording, stimulation, and LED illumination are controlled using an Arduino microcontroller. Chemical stimulation through the microfluidic arena switches between stimulus (S) and buffer (B) solutions via control fluid flow (C). <t>Brightfield</t> images are used to track sleep behavior and fluorescent images are used to measure GCaMP calcium transients in neurons. Image capture, sleep/wake state determination, and chemical stimulation are controlled by a computer in a closed loop, without user intervention.
    Illuminator Microled (Brightfield), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illuminator microled (brightfield)/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    illuminator microled (brightfield) - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation"

    Article Title: Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.101532

    Schematic of the closed-loop neural recording setup for sleep/wake response tracking Video recording, stimulation, and LED illumination are controlled using an Arduino microcontroller. Chemical stimulation through the microfluidic arena switches between stimulus (S) and buffer (B) solutions via control fluid flow (C). Brightfield images are used to track sleep behavior and fluorescent images are used to measure GCaMP calcium transients in neurons. Image capture, sleep/wake state determination, and chemical stimulation are controlled by a computer in a closed loop, without user intervention.
    Figure Legend Snippet: Schematic of the closed-loop neural recording setup for sleep/wake response tracking Video recording, stimulation, and LED illumination are controlled using an Arduino microcontroller. Chemical stimulation through the microfluidic arena switches between stimulus (S) and buffer (B) solutions via control fluid flow (C). Brightfield images are used to track sleep behavior and fluorescent images are used to measure GCaMP calcium transients in neurons. Image capture, sleep/wake state determination, and chemical stimulation are controlled by a computer in a closed loop, without user intervention.

    Techniques Used: Control

    Sample experiment settings file These sample settings define imaging resolution (bin 2), framerate (100 ms exposure = 10 frames/s), and illumination timing (20 ms for brightfield, 10 ms for fluorescence with 5 ms delay from exposure onset). Stimulation recordings capture 300 frames (or 30 s at 10 fps), and the stimulation program is a 10 s chemical pulse (valve 1 opens from frame 50–150 or from 5–15 s within each recording trial). The entire experiment lasts 12 h. Sleep state changes are monitored using a 10-s interval and changes must persist for 3 intervals (i.e., 30 s) to register a transition. After each stimulation trial, 15 min should elapse before triggering a new stimulation (regardless of any transitions within this time delay), and the next trigger shall begin following the opposite transition (i.e., Sleep-to-wake follows Wake-to-sleep).
    Figure Legend Snippet: Sample experiment settings file These sample settings define imaging resolution (bin 2), framerate (100 ms exposure = 10 frames/s), and illumination timing (20 ms for brightfield, 10 ms for fluorescence with 5 ms delay from exposure onset). Stimulation recordings capture 300 frames (or 30 s at 10 fps), and the stimulation program is a 10 s chemical pulse (valve 1 opens from frame 50–150 or from 5–15 s within each recording trial). The entire experiment lasts 12 h. Sleep state changes are monitored using a 10-s interval and changes must persist for 3 intervals (i.e., 30 s) to register a transition. After each stimulation trial, 15 min should elapse before triggering a new stimulation (regardless of any transitions within this time delay), and the next trigger shall begin following the opposite transition (i.e., Sleep-to-wake follows Wake-to-sleep).

    Techniques Used: Imaging, Fluorescence


    Figure Legend Snippet:

    Techniques Used: Virus, Expressing, Recombinant, Software, Control, Isolation, Fluorescence, Inverted Microscopy



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    Image Search Results


    Schematic of the closed-loop neural recording setup for sleep/wake response tracking Video recording, stimulation, and LED illumination are controlled using an Arduino microcontroller. Chemical stimulation through the microfluidic arena switches between stimulus (S) and buffer (B) solutions via control fluid flow (C). Brightfield images are used to track sleep behavior and fluorescent images are used to measure GCaMP calcium transients in neurons. Image capture, sleep/wake state determination, and chemical stimulation are controlled by a computer in a closed loop, without user intervention.

    Journal: STAR Protocols

    Article Title: Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation

    doi: 10.1016/j.xpro.2022.101532

    Figure Lengend Snippet: Schematic of the closed-loop neural recording setup for sleep/wake response tracking Video recording, stimulation, and LED illumination are controlled using an Arduino microcontroller. Chemical stimulation through the microfluidic arena switches between stimulus (S) and buffer (B) solutions via control fluid flow (C). Brightfield images are used to track sleep behavior and fluorescent images are used to measure GCaMP calcium transients in neurons. Image capture, sleep/wake state determination, and chemical stimulation are controlled by a computer in a closed loop, without user intervention.

    Article Snippet: Illuminator microLED (brightfield) , Zeiss , Cat #423053-9072.

    Techniques: Control

    Sample experiment settings file These sample settings define imaging resolution (bin 2), framerate (100 ms exposure = 10 frames/s), and illumination timing (20 ms for brightfield, 10 ms for fluorescence with 5 ms delay from exposure onset). Stimulation recordings capture 300 frames (or 30 s at 10 fps), and the stimulation program is a 10 s chemical pulse (valve 1 opens from frame 50–150 or from 5–15 s within each recording trial). The entire experiment lasts 12 h. Sleep state changes are monitored using a 10-s interval and changes must persist for 3 intervals (i.e., 30 s) to register a transition. After each stimulation trial, 15 min should elapse before triggering a new stimulation (regardless of any transitions within this time delay), and the next trigger shall begin following the opposite transition (i.e., Sleep-to-wake follows Wake-to-sleep).

    Journal: STAR Protocols

    Article Title: Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation

    doi: 10.1016/j.xpro.2022.101532

    Figure Lengend Snippet: Sample experiment settings file These sample settings define imaging resolution (bin 2), framerate (100 ms exposure = 10 frames/s), and illumination timing (20 ms for brightfield, 10 ms for fluorescence with 5 ms delay from exposure onset). Stimulation recordings capture 300 frames (or 30 s at 10 fps), and the stimulation program is a 10 s chemical pulse (valve 1 opens from frame 50–150 or from 5–15 s within each recording trial). The entire experiment lasts 12 h. Sleep state changes are monitored using a 10-s interval and changes must persist for 3 intervals (i.e., 30 s) to register a transition. After each stimulation trial, 15 min should elapse before triggering a new stimulation (regardless of any transitions within this time delay), and the next trigger shall begin following the opposite transition (i.e., Sleep-to-wake follows Wake-to-sleep).

    Article Snippet: Illuminator microLED (brightfield) , Zeiss , Cat #423053-9072.

    Techniques: Imaging, Fluorescence

    Journal: STAR Protocols

    Article Title: Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation

    doi: 10.1016/j.xpro.2022.101532

    Figure Lengend Snippet:

    Article Snippet: Illuminator microLED (brightfield) , Zeiss , Cat #423053-9072.

    Techniques: Virus, Expressing, Recombinant, Software, Control, Isolation, Fluorescence, Inverted Microscopy